Journal: Cancer Biology & Therapy

Article Title: MBD2 suppresses SFRP1 expression and promotes colorectal cancer development by blocking MED19 binding to its methylated promoter

doi: 10.1080/15384047.2026.2667568

Figure Lengend Snippet: The expression level and intracellular distribution of MED19 had no difference between normal cells/tissues and CRC cells/tissues. (A) The expression and distribution of MED19 protein in NCM460 cells and SW480 cells. (B) The expression and distribution of MED19 protein in normal colorectal tissues, adjacent colorectal cancer tissues, and colorectal cancer tumor tissues. (C) KCC07 did not affect the expression and distribution of MED19 in SW480 cells. n = 3, ns indicates not significant, *indicates p < 0.05, **indicates p < 0.01, and scale bar = 100 µm.

Article Snippet: Normal colon mucosa cell line NCM460 (ATCC, CRL-1642 TM ) and the human colorectal cancer cell lines HCT116 (ATCC, CCL-247 TM ) and SW480 (ATCC, CCL-228 TM ) were purchased from iCell, and all had STR identification reports.

Techniques: Expressing

Journal: Nature Communications

Article Title: Targeting NAT10 alleviates colonic senescence and elderly-onset colitis by disrupting N4-acetylation of DYRK1A

doi: 10.1038/s41467-026-70220-w

Figure Lengend Snippet: a Flow diagram of mRNA modification screening. m/z, mass-to-charge ratio. Created in BioRender. Chen, J. (2026) https://BioRender.com/h61m193 . b Analysis of SA-Gal activity in proliferation, H-Sen and D-Sen cells of NCM460 and CCD841. The SA-Gal - positive percentages in senescent cell groups were compared to the proliferation cell group. H-Sen, D-Sen: senescent cell induced by H 2 O 2 or DOX. Scale bar for NCM460, 100 µm. Scale bar for CCD841, 50 µm. n = 3 independent experiments. Data were represented as mean ± SD. c , d mRNA modification levels in proliferation cells and senescent cell groups of NCM460 and CCD841. n = 3 independent experiments. Data were represented as mean ± SD and represented relative to the control. e Dot blot of RNA ac 4 C modification level and western blot of Cyclin D1, γ-H2AX, p53, p21, p16 and NAT10 in senescent NCM460 and CCD841 cells. f Cell morphology and SA-Gal staining of P5 and P20 CCD841 cells. Scale bar, 50 µm. n = 3 independent experiments. Data were represented as mean ± SD. g mRNA ac 4 C modification level in P5 and P20 CCD841cells. n = 3 independent experiments. Data were represented as mean ± SD. Comparisons were performed by a Two-tailed unpaired Student’s t test ( b – d , f , g ). *** p < 0.001, ** p < 0.01, ns p > 0.05. Source data and exact p -value are provided as a Source data file.

Article Snippet: The colon epithelial cell line NCM460 (Cellosaurus, #305430, RRID: CVCL_0460) was cultured in RPMI 1640 medium (GIBCO, Waltham, MA, USA), and the finite colon cell line CCD841 (ATCC, CRL-1790, RRID: CVCL_2871) was maintained in Eagle’s minimum essential medium (ATCC, Manassas, VA, USA).

Techniques: Modification, Activity Assay, Control, Dot Blot, Western Blot, Staining, Two Tailed Test

Journal: Nature Communications

Article Title: Targeting NAT10 alleviates colonic senescence and elderly-onset colitis by disrupting N4-acetylation of DYRK1A

doi: 10.1038/s41467-026-70220-w

Figure Lengend Snippet: a Dot blot of RNA ac 4 C modification levels in NAT10-reexpressed (NAT10-WT or NAT10-MUT) NCM460 cells after transfected with NAT10-Si and treated with H 2 O 2 or DOX. n = 3 independent experiments. Data were represented as mean ± SD. b Western blot of NAT10, CyclinD1, γ-H2AX, p21, p53, and p16 in NAT10-reexpressed (NAT10-WT or NAT10-MUT) cells after transfected with NAT10-Si and treated with H 2 O 2 or DOX. c Analysis of SA-Gal activity and positive immunofluorescence staining of γ-H2AX in NAT10-reexpressed NCM460 cells after transfected with NAT10 siRNA and treated with H 2 O 2 . Scale bar for SA-Gal, 100 µm. Scale bar for IF, 20 µm. n = 3 independent experiments. Data were represented as mean ± SD. d Flow diagram for senescent cell viability and caspase3/7 activity detection. Created in BioRender. Chen, J. (2026) https://BioRender.com/w97m115 . e Colony formation assay using H-Sen NCM460 cells after co-transfection of NAT10-Si with either NAT10-WT or NAT10-MUT. f Cell viability analysis in H-Sen NCM460 cells after co-transfection of NAT10-Si with either NAT10-WT or NAT10-MUT. n = 3 independent experiments. Data were represented as mean ± SD. g Caspase3/7 activity analysis in H-Sen NCM460 cells after co-transfection of NAT10-Si with either NAT10-WT or NAT10-MUT. n = 3 independent experiments. Data were represented as mean ± SD. h Apoptosis flow cytometry analysis in H-Sen NCM460 cells after co-transfection of NAT10-Si with either NAT10-WT or NAT10-MUT. n = 3 independent experiments. Data were represented as mean ± SD. Comparisons were performed by Two-tailed unpaired Student’s t test ( a , c , f , g , h ). *** p < 0.001, ** p < 0.01, ns p > 0.05. Source data and exact p -value are provided as a Source data file.

Article Snippet: The colon epithelial cell line NCM460 (Cellosaurus, #305430, RRID: CVCL_0460) was cultured in RPMI 1640 medium (GIBCO, Waltham, MA, USA), and the finite colon cell line CCD841 (ATCC, CRL-1790, RRID: CVCL_2871) was maintained in Eagle’s minimum essential medium (ATCC, Manassas, VA, USA).

Techniques: Dot Blot, Modification, Transfection, Western Blot, Activity Assay, Immunofluorescence, Staining, Colony Assay, Cotransfection, Flow Cytometry, Two Tailed Test

Journal: Nature Communications

Article Title: Targeting NAT10 alleviates colonic senescence and elderly-onset colitis by disrupting N4-acetylation of DYRK1A

doi: 10.1038/s41467-026-70220-w

Figure Lengend Snippet: a Schematic illustration of acRIP-seq in NCM460 cells. Created in BioRender. Chen, J. (2026) https://BioRender.com/j92d013 . b Pie charts showed the distribution of ac 4 C peak in the acetylated transcripts in the control NC-Si, H-Sen NC-Si, and H-Sen NAT10-Si groups. c GO-BP enrichment analysis of hyperacetylated genes in H-Sen NCM460 cells compared to control NCM460 cells (left). GO-BP enrichment analysis of hypoacetylated genes in H-Sen NCM460 cells with NAT10-Si compared to H-Sen NCM460 cells with NC-Si (right). d Overlapping the potential downstream targets of NAT10-meditated RNA ac 4 C modification in hyperacetylated gene (H-Sen NC-Si vs Control NC-Si) and hypoacetylated gene (H-Sen NAT10-Si vs H-Sen NC-Si) by the acRIP-seq analysis. e acRIP-qPCR validation of ac 4 C level alterations in four potential downstream targets across the control NC-Si, H-Sen NC-Si, and H-Sen NAT10-Si groups. n = 3 independent experiments. Data were represented as mean ± SD. f qPCR was used to validate DYRK1A and LMLN RNA expression across the three groups. n = 3 independent experiments. Data were represented as mean ± SD. g Actinomycin D (ActD) chase RNA-seq showing DYRK1A mRNA level change in H-Sen NC-Si and H-Sen NAT10-Si cells. n = 3 independent samples. Data were represented as mean ± SD. h IGV plots showed that ac 4 C peaks changes in the CDS domains of DYRK1A mRNA in the control NC-Si, H-Sen NC-Si, and H-Sen NAT10-Si groups from acRIP-seq data. i RNA ac 4 C modifications and THAP5, DYRK1A and LMLN protein levels were detected by dot blot or western blot across the three groups. Two-way ANOVA analysis with Tukey’s multiple comparison was used for ( g ). Two-tailed unpaired Student’s t test was used for other analyses ( e , f ). *** p < 0.001, ** p < 0.01, ns p > 0.05. Source data and exact p -value are provided as a Source data file.

Article Snippet: The colon epithelial cell line NCM460 (Cellosaurus, #305430, RRID: CVCL_0460) was cultured in RPMI 1640 medium (GIBCO, Waltham, MA, USA), and the finite colon cell line CCD841 (ATCC, CRL-1790, RRID: CVCL_2871) was maintained in Eagle’s minimum essential medium (ATCC, Manassas, VA, USA).

Techniques: Control, Modification, Biomarker Discovery, RNA Expression, RNA Sequencing, Dot Blot, Western Blot, Comparison, Two Tailed Test

Journal: Nature Communications

Article Title: Targeting NAT10 alleviates colonic senescence and elderly-onset colitis by disrupting N4-acetylation of DYRK1A

doi: 10.1038/s41467-026-70220-w

Figure Lengend Snippet: a acRIP-qPCR analysis of ac 4 C levels in the CDS domain of DYRK1A among control, H-Sen, and D-Sen NCM460 cells. n = 3 independent experiments. Data were represented as mean ± SD. b DYRK1A mRNA expression and protein expression in control, H-Sen, and D-Sen NCM460 cells. n = 3 independent experiments. Data were represented as mean ± SD. c Immunofluorescence staining of DAPI, γ-H2AX, and DYRK1A in control, H-Sen, and D-Sen NCM460 cells. Scale bar for immunofluorescence, 20 μm. d Immunoblotting of NAT10 after NAT10-RIP assay with cell lysate among control, H-Sen, and D-Sen NCM460 cells. qPCR analysis of the CDS domain of DYRK1A was performed after NAT10-RIP. n = 3 independent experiments. Data were represented as mean ± SD. e DYRK1A mRNA stability was measured by adding actinomycin D in control, H-Sen, D-Sen NCM460 cells and in H-Sen and D-Sen NCM460 cells with NAT10 knockdown. n = 3 independent experiments. Data were represented as mean ± SD. f Sucrose gradient fractionation of H-Sen NCM460 cells and H-Sen NCM460 cells with NAT10 knockdown. Relative DYRK1A mRNA abundance across the polysome fraction was quantified by RT-qPCR. n = 3 independent experiments. Data were represented as mean ± SD. g Analysis of SA-Gal activity in DYRK1A-reexpressed NCM460 cells after transfected with NAT10 siRNA and treated with H 2 O 2 or DOX. Scale bar for SA-Gal, 100 µm. h qPCR analysis of IL-1β and IL-6 in DYRK1A-reexpressed NCM460 cells after transfected with NAT10 siRNA and treated with H 2 O 2 or DOX. n = 3 independent experiments. Data were represented as mean ± SD. i Western blot of NAT10, DYRK1A, γ-H2AX, and p21 in DYRK1A-reexpressed cells after transfected with NAT10-Si and treated with H 2 O 2 or DOX. j Caspase3/7 activity analysis in DYRK1A-reexpressed H-Sen and D-Sen NCM460 cells after transfected with NAT10-Si. n = 3 independent experiments. Data were represented as mean ± SD. Two-way ANOVA analysis with Tukey’s multiple comparison was used for ( e ). Two-tailed unpaired Student’s t test was used for other analyses ( a , b , d , e , f , g , h , j ). *** p < 0.001, ** p < 0.01, * p < 0.05. Source data and exact p -value are provided as a Source data file.

Article Snippet: The colon epithelial cell line NCM460 (Cellosaurus, #305430, RRID: CVCL_0460) was cultured in RPMI 1640 medium (GIBCO, Waltham, MA, USA), and the finite colon cell line CCD841 (ATCC, CRL-1790, RRID: CVCL_2871) was maintained in Eagle’s minimum essential medium (ATCC, Manassas, VA, USA).

Techniques: Control, Expressing, Immunofluorescence, Staining, Western Blot, Knockdown, Fractionation, Quantitative RT-PCR, Activity Assay, Transfection, Comparison, Two Tailed Test